Regeneration and transformation potential of elite Kenyan highland maize inbred lines
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Date
2010
Authors
Machuka, Jesse
Taracha, C.
Nangayo, F.
Ombakho, G.
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Abstract
Agrobacterium-mediated transformation of tropical maize has been manipulated in only a limited number of genotypes, because a majority of maize germplasm isrecalcitrant toin vitroresponse. Establishment of a highly efficiency and widely used tissue culture system for maize will accelerate the application of transformation technology in breeding programs, and the study of the functions of maize specific genes. Out of the three media evaluated, it was established that two media could guarantee the production and proliferation of a large number of embyogenic calli with high regeneration capacityfrom immature zygotic embryos representing different maize germplasm. The results suggest that the evaluated tissue system will be widely applicable for the tissue culture of Elite Kenyan Highland inbred maize lines
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Keywords
Embryogenic calli, mmature zygotic embryos, Agro bacterium-mediated transformation, maize germplasm
Citation
Proceedings of The 12TH KARI Biennial Scientific Conference “Transforming Agriculture for improved livelihoods through Agricultural Product Value Chains”