RP-Department of Medical Physiology

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    Streptococcus pneumoniae serotype epidemiology among PCV-10 vaccinated and unvaccinated children at Gertrude’s Children’s Hospital, Nairobi County: a cross-sectional study
    (F1000Research, 2018) Walekhwa, Michael; Muturi, Margaret; Gunturu, Revathi; Kenya, Eucharia; Kabera, Beatrice
    Background: Streptococcus pneumoniae (SPn) serotype replacement and emergence of multidrug resistant SPn has exacerbated the need for continuous regional serotype surveillance. We investigated SPn serotypes circulating among children ≤5 years in Nairobi County. Methods: Streptococcus pneumoniae stocks stored at −70°C in brain heart infusion medium were thawed at room temperature for 30 minutes. In total, 10 μl of the stored SPn cells were suspended in 50 μl PBS and gently vortexed. About 10 μl of the suspended cells were added on to a glass slide and mixed with 10 μl pooled antisera. The glass slide was swirled gently while observing for any reaction. The process was repeated with individual groups under various antisera pools. Those serotypes that did not belong to any pool were typed directly until a positive agglutination reaction was observed. The cells/PBS/serotype-specific antisera mixture on the glass slide were covered with a coverslip and observed under a phase contrast microscope at ×100 objective lens with oil emulsion. Results: Out of the 206 subjects sampled, 20.39% (n=42) were found to be carriers of SPn. About 52% (n=22) of the SPn carriers had received the recommended dose of PCV-10, while 48% (n=20) of the carriers had not. Almost all (n=41; 19.90% of subjects) isolates contained non-vaccine type SPn serotypes, while n=1 of the serotypes (in 0.49% of subjects) were untypeable. Serotypes 28F, 6A, 11A, 3 and 7C were prevalent in both vaccinated and unvaccinated children, whereas serotypes 23A, 17F, 35F, 48, 13 and 35B, and 23B, 20, 19B, 21, untypeable, 15B and 39 were found among unvaccinated and vaccinated groups, respectively. Conclusions: All SPn serotypes isolated from the subjects sampled were non PCV-10 vaccine type. Therefore Kenyan children receiving PCV-10 vaccine are not protected.