Browsing by Author "Ngugi, Mathew"

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    Ficus Sycomorus: Ecological, Cultural, and Medicinal Insights into a Timeless Tree
    (A Multidisciplinary peer-reviewed Journal, 2025-02) Muthee, Eunice Wothaya; Ngugi, Mathew; Gitahi, Stephen
    Ficus sycomorus, commonly known as the sycamore fig, is a large deciduous tree native to tropical and subtropical regions of Africa, the Mediterranean, and the Middle East. It plays a crucial ecological role in riparian zones, supporting biodiversity and preventing soil erosion. Historically, the tree has held significant cultural and spiritual importance, particularly in ancient Egypt and various African cultures, where it symbolizes fertility, protection, and nourishment. Medicinally, Ficus sycomorus has been utilized for centuries, with various parts of the tree—such as the fruit, leaves, bark, and latex—employed in the treatment of numerous ailments, including gastrointestinal disorders, respiratory issues, wounds, and diabetes. The medicinal properties of the tree are attributed to its rich phytochemical composition, which includes flavonoids, tannins, alkaloids, and phenolic compounds, responsible for its antioxidant, antiinflammatory, antimicrobial, and antidiabetic activities. The anti-inflammatory effects of Ficus sycomorus, in particular, have been highlighted in scientific studies, demonstrating its potential in managing chronic inflammatory diseases through mechanisms such as the inhibition of pro-inflammatory cytokines, enzymes, and oxidative stress. Despite threats from habitat destruction, the tree's resilience and ongoing cultural and medicinal relevance underscore the importance of its conservation and further research. This paper explores the ecological, cultural, and medicinal significance of Ficus sycomorus, highlighting its multifaceted contributions to both nature and human well-being.
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    In Vitro Anti-Prostate Cancer Efficacy and Phytochemical Composition of the Dichloromethane and Ethyl Acetate Leaf Extracts Of Vitex Doniana (Sweet)
    (Frontiers in Pharmacology, 2024-11) Moriasi, Gervason; Ngugi, Mathew; Mwitari, Peter
    Background: Prostate cancer is a significant global health concern, particularly among ageing male populations, with a disproportionately higher burden in subSaharan Africa. Conventional treatments, though effective, are costly and cause devastating side effects which limit their clinical benefits. Hence, this study evaluated the in vitro antiprostate cancer properties and secondary metabolites of dichloromethane and ethyl acetate lead extracts of Vitex doniana to explore safer and efficacious natural alternatives based on ethnomedicinal claims. Methods: Phytochemical profiling was conducted using gas chromatographymass spectrometry (GC-MS) analysis to identify secondary metabolites in the extracts. The cytotoxic effects of the extracts were determined through the MTT assay using Vero CCL-81 cells and DU-145 cells. The expression profile of the selected genes (ar, bcl2, caspase-3, cdk1, and p53) in DU-145 cells treated with the study extracts was investigated using RT-qPCR. Results: GC-MS analysis revealed 10 secondary metabolites in the dichloromethane extract and 27 secondary metabolites in the ethyl acetate extract of V. doniana leaves, with the majority being sesquiterpenes, diterpenoids, and phytosterols. The dichloromethane and ethyl acetate leaf extracts of V. doniana exhibited low cytotoxicity against normal mammalian epithelial cells (Vero CCL-81), with CC50 values of 1,238.85 μg/mL and 964.81 μg/ mL, respectively. Besides, the ethyl acetate leaf extract of the studied plant demonstrated potent anti-prostate cancer activity against DU-145 cells, with an IC50 of 35.68 μg/mL and a high selectivity index (SI) of 27.04. Likewise, the dichloromethane leaf extract of this plant displayed cytotoxic effects (IC50: 287.01 μg/mL) and a selectivity index of 4.32. The reference drug (Doxorubicin) showed a higher toxicity against Vero CCL-81(IC50: 0.41 μg/mL) and DU-145 (IC50: 0.28 μg/mL) cells and a lower selectivity index of 1.46. The DU145 cells treated with the studied plant extracts exhibited notable upregulation of ar and bcl2, and normalization of caspase 3, cdk1 and p53 expression. Conclusion: The studied plant extracts possess in vitro anti-prostate cancer properties and could be promising candidates for further preclinical studies aimed at developing novel botanical-based therapies for the management of prostate cancer.
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    Warburgia Ugandensis and Croton Dichogamus: Possible Botanical Bullets against Callosobruchus Maculatus
    (A Multidisciplinary peer-reviewed Journal, 2025) Katoo, Athanas Alexander; Ngugi, Mathew; Gitahi, Stephen
    Botanical pesticides, derived from plant products, have gained significant popularity as an alternative to conventional synthetic insecticides. These natural products often consist of various bioactive compounds that offer effective pest control and may reduce the chances of pest resistance. However, challenges such as variation in pest response, short residual activity, and difficulties in large-scale production hinder their widespread use. This paper explores different generations of botanical pesticides, focusing on their chemical composition, modes of action, and applications. First generation botanical pesticides, including nicotine, rotenone, essential oils, sabadilla, and pyrethrum, are discussed, along with their associated limitations. The second-generation botanical pesticides, such as Melia extracts, synthetic pyrethroids, and neem products, are presented as more specific and environmentally friendly alternatives. Furthermore, the insecticidal potential of Croton dichogamus and Warburgia ugandensis, two East African plants, is examined. Both plants possess promising phytochemical properties that show potential for controlling pests like Callosobruchus maculatus in stored grains. This review highlights the ecological and economic value of these plants in pest management strategies and the sustainable use of plant-based pesticides.