PHD-Department of Zoologial Sciences

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Browsing PHD-Department of Zoologial Sciences by Author "Bruno, Le Ru"

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    Diversity and ecological preference of parasitoids associated with lepidopteran stem borers in Kenya
    (2011-08-03) Mailafiya, D. M.; Kairu, E. W.; Bruno, Le Ru; Dupas, S.
    Field surveys were carried out from 2005 to 2007 to assess parasitoid species diversity and stemborer parasitism in cultivated and natural habitats in four agroecological zones in Kenya. To increase the chances of collecting stemborer parasitoids, random sampling was employed in cultivated habitats, while both random and non-random samplings were applied in natural habitats. During this study, 7443 and 3676 stemborers were collected from cultivated and natural habitats, respectively. A total of 33 parasitoid species were recorded, of which 18 species were recovered from 6 stemborer species feeding on cereal crops, while 27 species were recovered from 21 stemborer species feeding on 19 wild host plant species. The most common parasitoid species in cultivated habitats were Cotesia flavipes (Hymenoptera: Braconidae), C. sesamiae (Hymenoptera: Braconidae), Pediobius furvus (Hymenoptera: Bethylidae) and Siphona sp. (Diptera: Tachinidae). In natural habitats, Siphona sp. was the most common. Parasitoid species diversity was significantly higher in natural than in cultivated habitats. Most of the parasitoid species recovered from cultivated habitats were also found in natural habitats. Natural habitats surrounding cereal crops appeared to serve as refugia for sustaining the diversity of stemborer parasitoids in adjacent cereal fields. Given that parasitoid diversity was higher in natural habitats with more diverse plant communities, but parasitoid abundance was higher in cultivated habitats, this study provided partial support for the `natural enemy' hypothesis. Across agroecological zones, stemborer parasitism was positively dependent on parasitoid species richness and abundance during different seasons in both cultivated and natural habitats. Rainfall had a significant negative effect on parasitoid abundance as well as stemborer parasitism in both cultivated and natural habitats. The negative relationship between parasitism and temperature in natural habitats only, suggested greater sensitivity of parasitoids to extreme temperatures in natural habitats. Across seasons, stemborer densities were generally higher on maize and sorghum in cultivated habitats than on wild host plants in . natural habitats. Stemborer parasitism rates were generally low (<15.00%) in both cultivated and natural habitats. Neither larval nor pupal parasitism differed significantly between seasons in natural habitats. Cotesia sesamiae was found in Kakamega and Suam in the Western and Rift Valley regions, respectively, where Busseola fusca (Lepidoptera: Noctuidae) predominates. By contrast, C. flavipes was found in Mtito Andei and Muhaka in the Eastern and Coastal regions, respectively, where Chilo partellus (Lepidoptera: Pyralidae) predominates. There was no indication of host plant and stemborer species specificity by C. sesamiae, as its host ranges varied with both habitat type and locality. Conversely, C. flavipes exhibited a high level of specificity to its target host C. partellus on maize and sorghum in cultivated habitats and on S. arundinaceum in natural habitats. Of the two Cotesia species, natural habitats served as a reservoir for C. flavipes only, because these habitats sustained some of the individuals on C. partellus during both rainy and dry seasons. This was however not applicable to C. sesamiae, as its cereal stemborer hosts B. fusca and Sesamia calamistis (Lepidoptera: Noctuidae) were scarce in wild host plants.
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    Diversity, Ecology and population dynamics of Lepidopteran stem borers in Kenya
    (Kenyatta University, 2009-07) Ong'amo, George Otieno; Kokwaro, Elizabeth D.; Silvan, Jean-Francois; Bruno, Le Ru
    Stem borers are important field insect pests of maize [Zea mays L.] and sorghum [Sorghum bicolor (L.) Moench] in Africa. They account for more than 30% yield losses depending on fhe composition of the pest community. A total of 21 pest species have been reported in sub-Saharan Africa all of which are indigenous to the continent except for Chilo partellus (Swinhoe), which was accidentally introduced from Asia. Stem borers are susceptible to environmental fluctuations and the pest species are thought to have experienced changes in physiology and behaviour after close association with highly nutritive crops. Recent studies indicate that in addition to the pest species, there are noneconomic stem borer species among wild hosts in the uncultivated fragments. Owing to susceptibility of stem borer species, continued habitat fragmentation and degradation may ultimately result in host range expansion and eventual emergence of "new" pests. Unfortunately, previous studies have been geared towards reducing populations of pest species in the cultivated fields with few attempts to understand possible evolution of less known species to pest status. This research was therefore designed to gather information on stem borer species diversity, host range and ecology in selected agricultural landscapes in Kenya. Surveys were conducted during 2005/2007 growing seasons in and around selected cultivated fields in four localities, Muhaka, Mtito Andei, Kakamega and Suam, representing different altitudinal gradients across the country. A total of 29 stem borer species were identified from 9,771 larvae collected. The identified stem borer species were grouped into 10 different known genera (Acrapex, Busseola, Carelis, Manga, Poecopa, Sciomesa, Sesamia, Eldana, Chilo and Ematheudes) while the unknown species belonged to five different families (Crambidae, Peoriinae, Pyralidae, Schoenobiinae and Tortricidae). There was evidence of variation in both distribution and dominance among the surveyed localities with majority of the species belonging to the Noctuidae family found in Kakamega and Suam, while species belonging to Crambidae and Pyralidae were mainly found in Muhaka and Mtito Andei. The wild stem bore species were identified from 38 different plant species belonging to three different families (Cyperacea [27], Poaceae [10] and Typhaceae [1]), while pest species, Busseola fusca (Fuller), Sesamia calamistis Hampson, Chilo partellus Swinhoe, Chilo orichalcociliellus (Strand) and Eldana saccharina Walker were mainly found on maize and sorghum. Sesamia calamistis and B. phaia ssp. phaia occurred among both wild and cultivated hosts and provided good models for studying exchange of stem borer pest populations between the wild and cultivated habitats. Cytochrome b gene sequences, through the existence of strong genetic structuration, revealed evidence of limited exchange of S. calamistis populations between the habitats. However, genetic analyses of the same gene of Busseola phaia ssp. phaia Bowden populations revealed weak differentiation with respect to host use in different habitats (FsT = 0.016; P = 0.015). Observed variations in the distribution of pest and non-pest stem borer species coupled with differences in genetic structure among model species (S. calamistis and B. phaia ssp. phaia) suggest two things; i) no single management strategy would apply across different landscapes and ii) continued habitat fragmentation 1 degradation would affect ecosystem stability resulting in host range expansion or local species extinctions. Similar intensive studies need to be extended to other areas as it will form the basis upon which different integrated pest management (IPM) packages could be developed.