RP-Department of Foods, Nutrition & Dietetics

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Browsing RP-Department of Foods, Nutrition & Dietetics by Author "Apostolides, Z."

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    Effects of processing technique on the nutritional composition and anti-nutrient content of mucuna bean (Mucuna pruriens L.)
    (Academic Journals, 2010-04) Mugendi, Jane Beatrice; Njagi, E.N.M.; Kuria, E. N.; Mwasaru, M. A.; Mureithi, J. G.; Apostolides, Z.
    Mucuna bean (Mucuna pruriens) is an indigenous legume promoted in Kenya as a green manure cover crop. It contains high protein but it is under - utilized due to the presence of 3, 4-dihydroxy-Lphenylalanine (L-Dopa) and other anti nutritional compounds. To improve its nutritional potential as a protein source, mucuna bean was processed and evaluated for nutritional composition. Effects of processing at different pH, temperature and particle size, autoclaving, germination and fermentation on the contents of anti-nutritional compounds and crude protein were investigated. Raw beans contained high crude protein (27.9 g/100-1). Contents of ether extract; crude fibre and ash were 3.7, 7.9 and 3.5 g/100-1, respectively. Mineral content was comparable to that of common pulses. Raw whole mucuna bean contained high L-Dopa (7.0 g/100-1) content. Other anti-nutritional compounds included total phenols 7.1 g/100-1, trypsin inhibitor activity (TIA) 5.1 TIU and phytates 0.9 g/100-1. All processing techniques, except roasting, reduced levels of L-dopa by > 95% while less than 15% of protein was lost. Soaking dehulled bean in acidic medium (pH 3.2) at 60°C for 48 h reduced L-Dopa content to the recommended safe level of 0.1%. Processing mucuna bean increased its nutritional value and potential to improve food security.
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    Nutritional quality and physicochemical properties of Mucuna bean (Mucuna pruriens L.) protein isolates
    (Faculty of Food Science and Technology, 2010) Mugendi, Jane Beatrice; Njagi, E.N.M.; Kuria, E. N.; Mwasaru, M. A.; Mureithi, J. G.; Apostolides, Z.
    The potential for mucuna bean protein isolate (MBPI) application as functional ingredient in foods is unknown. In this study nutritional quality and physicochemical properties of MBPI were investigated. Bean samples were processed for L-dopa extraction in distilled water adjusted to pH 3.2 at 60°C for 48hr. MBPI was extracted at pH 9.0 and isoelectrically precipitated at pH 4.5. MBPI from raw and processed seed contained higher protein content (86.7 and 86.9% respectively) than soybean protein isolate (82.7%). Essential amino acids content of MBPI met FAO/WHO scoring pattern for 2-5 year-old. SDS-PAGE revealed four main polypeptide protein subunits of apparent MW of 11, 19, 36 and 98 kD in MBPI. MBPI exhibited high foam stability, emulsion activity and stability compared to soybean protein isolate. However, poor foam expansion, water and oil absorption capacity and dark colour (Hunter lab “L” value of 36.39) limits its potential as a functional food ingredient
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    Nutritional quality and physicochemical properties of Mucuna bean (Mucuna pruriens L.) protein isolates
    (Elselvier, 2010) Mugendi, Jane Beatrice; Njagi, E.N.M.; Kuria, E. N.; Mwasaru, M.A.; Mureithi, J.G.; Apostolides, Z.
    The potential for mucuna bean protein isolate (MBPI) application as functional ingredient in foods is unknown. In this study nutritional quality and physicochemical properties of MBPI were investigated. Bean samples were processed for L-dopa extraction in distilled water adjusted to pH 3.2 at 60°C for 48hr. MBPI was extracted at pH 9.0 and isoelectrically precipitated at pH 4.5. MBPI from raw and processed seed contained higher protein content (86.7 and 86.9% respectively) than soybean protein isolate (82.7%). Essential amino acids content of MBPI met FAO/WHO scoring pattern for 2-5 year-old. SDS-PAGE revealed four main polypeptide protein subunits of apparent MW of 11, 19, 36 and 98 kD in MBPI. MBPI exhibited high foam stability, emulsion activity and stability compared to soybean protein isolate. However, poor foam expansion, water and oil absorption capacity and dark colour (Hunter lab “L” value of 36.39) limits its potential as a functional food ingredient.