Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid.
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Date
1970
Authors
Igambi, L.
Mandel, M.
Bergendahl, J.
Dodson, M. L. Jr.
Scheltgen, E.
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Volume Title
Publisher
American Society for Microbiology
Abstract
Samples of bacterial deoxyribonucleic acid from bacteria having guanine plus cytosine (GC) contents in the range of 27 to 72 moles per cent GC were analyzed by optical melting (T(m)) and equilibrium buoyant density methods. The relation between these properties is shown to be linear. The relative value of 1.99 moles per cent GC per degree C change in T(m) is calculated, and a reference method for the calculation of GC contents relative to a standard is derived.
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Citation
Journal of Bacteriology (Impact Factor: 3.19). 03/1970; 101(2):333-8. Source: PubMed