Effect of 2, 4-d levels on callus induction of leaf and stem explants of 5 local farmer-preferred sweet potato varieties in Kenya
Gichuki, S. T.
MetadataShow full item record
Five local and popular sweet potato (Ipomoea batatas(Lam.) varieties were selected from the major sweet potato growing areas, based on farmer-preferences and desirable characteristics. Both leaf and stem explants were incubated for callus induction with various concentrations of an auxin 2,4 Dichlorophenoxyacetic acid (2,4-D) at 0, 0.5, 1.0, 2.0, and 5.0 mg/L. The highest percentage of callus induction was realized at 2.0 mg/L and 5.0 mg/L 2,4-D in all varieties for both leaf and stem explants. Callus was induced at 3 weeks after incubation of leaf and stem explants in all tested varieties. Stem explants demonstrated a better response to callus induction at all 2,4-D concentrations and in all varieties as compared to leaf explants. All varieties responded differently to callus induction.