Population specific reference ranges of CD3, CD4 and CD8 lymphocyte subsets among healthy Kenyans
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Date
2013
Authors
Bosire, Erick M.
Nyamache, A. K.
Gicheru, M. M.
Samoel, A. Khamadi
Lihana, Raphael W.
Okoth, Vincent
Journal Title
Journal ISSN
Volume Title
Publisher
PMC
Abstract
Background: The enumeration of absolute CD4 counts is of primary importance for many medical conditions
especially HIV infection where therapeutic initiation depends on the count. These ranges tend to vary across
populations. However, these ranges have not been comprehensively established in the Kenyan population.
Therefore, this study aimed at establishing the reference ranges for the CD4 and CD8 T-lymphocytes in normal
healthy individuals in Kenya.
Methods: A total of 315 individuals of the ages between 16 and 60 years old, in 5 different regions of the country,
were recruited into the study. They were screened for diseases that potentially cause lymphocyte homeostasis
perturbation. CD4/CD8 Counts were performed by use of a FACSCalibur flow cytometer (Becton-Dickinson, NJ)
equipped with automated acquisition and analysis software. Results were analysed according to age, sex and region.
Results: Results were presented as means and ranges (in parenthesis) generated non parametrically as 2.5 and 97.5
percentiles as follows; In general population; CD3 1655 (614-2685 cells/μL ), CD4 920 (343-1493 cells/μL), and CD8 646
(187-1139 cells/μL), while according to sex, females; CD3 1787 (697-2841 cells/μL), CD4 1010 (422-1572 cells/μL), CD8
659 (187-1180 cells/μL); males; CD3 1610 (581-2641 cells/μL), CD4 889(320-1459 cells/μL) and CD8 644 (185-1140 cells/μL).
The general reference ranges for CD4/CD8 ratios were as follows; general population 1.57(0.50-2.74), males 1.51(0.49-2.64)
and females 1.69(0.55-2.95).
Conclusion: The lymphocyte reference ranges for the Kenyan population are fairly comparable to those established in
other African populations. The ranges also differ appreciably from those established in Germany, Italy and Switzerland.
Furthermore, the study reported significant differences in the ranges of different population clusters within Kenya, as well
us between males and females
Description
Keywords
CD4/CD8 count, Lymphocyte subsets, HIV-1, Flow cytometry, Reference ranges
Citation
AIDS Research and Therapy 2013, 10:24