In vitro regeneration of selected commercial Tanzanian open pollinated maize varieties
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Date
2012-03
Authors
Seth, Miccah Songelael
Bedada, Leta Tulu
Mneney, Emmarold E.
Oduor, R.O.
Machuka, Jesse
Journal Title
Journal ISSN
Volume Title
Publisher
Academic Journals
Abstract
Four Tanzanian open pollinated maize varieties namely; Kito, Situka M-1, Staha and TMV-1 were
regenerated in vitro using immature zygotic embryos as ex-plants. Callus induction was achieved using
Murashige and Skoog (MS) basal medium supplemented with 1, 1.5, 2 or 2.5 mg/l of 2, 4-D. Callus
induction was significantly affected by the genotype of the varieties. Among the varieties tested, Kito,
Situka M-1 and Staha had significantly higher callus induction frequency of 84.4, 92.7 and 88.7%,
respectively compared to TMV-1 (43.3%). Embryogenic callus induction percentage was significantly
influenced by the genotype, 2, 4-D concentrations and their interaction effect implying differential
response of the genotypes to 2, 4-D concentrations. Among the genotypes tested, Kito gave
significantly (p<0.05) the highest embryogenic callus frequency of 69.5%. Great variations were
observed among the genotypes with regard to regeneration frequencies. Staha was comparatively the
most regenerable variety with RF of 32.6% than even Kito which had the highest callus induction and
embryogenic callus formation. MS medium amended with 1 mg/l 2, 4-D was observed to be the optimum
formulation for the induction of embryogenic calli for genotypes tested in this study. Using this
regeneration system Situka M-1, Staha and TMV-1 can now be improved against various production
constraints through genetic engineering.
Description
Publisher version (Academic Journals Online) available at www.academicjournals.org/ajb/PDF/.../Seth%20et%20al.pdf
Keywords
2, 4-dichlorophexyacetic acid, embryogenic callus, immature zygotic embryos, regeneration frequency, somatic embryos, Tanzanian open pollinated maize
Citation
African Journal of Biotechnology Vol. 11(22), pp. 6043-6049, 15 March, 2012