Immunochemical detection of peanut (Arachis hypogaea L.) proteins using chicken antibodies
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Date
2012
Authors
Kiio, J.
De Meulenaer, B.
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor & Francis
Abstract
ELISA is a cheap, fast and simple method for the detection of food allergens. Use
of chicken antibodies in the analysis of food allergens and specifically the effect of
the immunisation frequency on antibody generation is not well documented. This
study aimed at comparing the activity of the immunoglobulins generated using a
three-week and a six-week immunisation protocol. Isa brown chicken were
immunised with 500 mg of roasted peanut protein extract using varying booster
injection intervals. After induction, antibody production was sustained during the
immunisation period in the six-week protocol with a marked drop in the threeweek
regime. The activity of antibodies was higher in the six-week protocol than
the three-week protocol. An assay was optimised in the detection range of 1 ppm.
Upon heating, the peanut protein binding by IgYs decreased with subsequent
fluctuations. This was associated with denaturation of proteins and unmasking of
new epitopes and stable fractions persisting.
Description
Publisher version (Taylor & Francis) available at http://dx.doi.org/10.1080/09540105.2011.615061
Keywords
detection, immunisation, immunoglobulins, peanut, protein
Citation
Food and Agricultural Immunology Vol. 23, No. 3, September 2012, 217 226