Neutralizing antibody responses in Africa green monkeys naturally infected with simian immunodeficiency virus (SIVagm)
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Date
2014-09-15
Authors
Gicheru, M. M.
Otsyula, M.
Spearman, P.
Graham, B.S.
Miller, C.
Robinson, H.L.
Haigwood, N.L.
Montefiori, D.C.
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Medical Primatology
Abstract
This study assessed the magnitude and cross-reactivity of the neutralizing antibody response generated by natural SIV infection in wild-caught African green monkeys. Neutralizing antibodies of variable potency, sometimes exceeding a titer of 1:1,000, were detected in 20 of 20 SIV-seropositive African green monkeys in Kenya. Detection of
those neutralizing antibodies was dependent on the strain of virus and the cells used for assay, where the most sensitive detection was made with SIVagm1532 in Sup T1 cells. Potent neutralization of SIVagm1532 was seen with contemporaneous autologous serum. Potent neutralization was also detected with laboratory-passaged SIVmac25 1 and SIVsmB670, but not with SIVsmE660 and two additional strains of SIVagm. Serum samples from rhesus macaques (Macaca mulatta) experimentally infected with either SIVmac25 1 or SIVsmE660 were capable of low-level neutralization of SIVagm. These results indicate that natural infection with SIV can generate strain-specific neutralizing antibodies in African green monkeys. They also indicate that some neutralization
determinants of SIVagm are partially shared with SIV strains that arose in sooty mangabys and were subsequently transmitted to 1 rhesus macaques.
Description
Research Paper
Keywords
antibodies - neutralization, nonhuman primates
Citation
Journal of Medical Primatology 1999: 28: 97-104