Hassanali, AhmedOmole, R. A.Machocho, Alex K.Ndiege, I. O.Gathirwa, JeremiahAkala, HoseaMalebo, Hamisi M.2014-11-252014-11-252014-07Phytochemistry Volume 103, July 2014, Pages 123–1280031-9422http://www.researchgate.net/publication/261564919_Bisbenzylisoquinoline_and_hasubanane_alkaloids_from_Stephania_abyssinica_(Dillon__A._Rich)_(Menispermeceae)1873-3700http://ir-library.ku.ac.ke/handle/123456789/11730doi:10.1016/j.phytochem.2014.03.026Two bisbenzylisoquinoline and one hasubanane alkaloids: (−)-pseudocurine (1), (−)-pseudoisocurine (2) and (−)-10-oxoaknadinine (3), were isolated from leaf extract of Stephania abyssinica, a plant used in traditional medicine in South Nyanza region of Kenya. They were characterized using 1D (1H, 13C and DEPT) and 2D (COSY, NOESY, HMQC and HMBC) NMR techniques. (−)-Pseudocurine (1) and (−)-pseudoisocurine (2) exhibited strong to moderate anti-plasmodial activity while (−)-10-oxoaknadinine (3) showed moderate to mild activity. ________________________________________ Graphical abstract From the leaves of Stephania abyssinica, three alkaloids were isolated: (−)-pseudocurine (1), (−)-pseudoisocurine (2) and (−)-10-oxoaknadinine (3). (−)-Pseudocurine and (-)-pseudoisocurine showed strong anti-plasmodial activity against both chloroquine-susceptible D6 and resistant strains of Plasmodium falciparum (IC50 0.29 ± 0.00 and 0.31 ± 0.01 μg/ml, respectively) while (−)-pseudoisocurine exhibited moderate and mild activity (0.75 ± 0.11 and 1.65 ± 0.03 μg/ml).enMenispermerceaeStephania abyssinicaAlkaloidsBisbenzylisoquinoline(−)-Pseudocurine and (−)-pseudoisocurineHasubanane(−)-10-oxoaknadinine anti-plasmodial activityArticle