Musili,PurityMuturi,MargaretMenza,Nelson2025-04-232025-04-232025Musili, P., Muturi, M. and Menza, N. (2025) Determination Antibiotic Sensitivity Profile of Bacterial Isolates among Reproductive Aged NonPregnant Women Attending Thika Level 5 Hospital. Open Access Library Journal, 12: e9794. https://doi.org/10.4236/oalib.11097942333-9721https://ir-library.ku.ac.ke/handle/123456789/29977ArticleIntroduction: Bacteriuria is commonly found in women and about thirty three percent (33%) of adult women have encountered an incidence of symptomatic cystitis at least once in their life time. In case the uropathogens are unidentified and management undertaken, bacteriuria may cause more dangerous and adversed complications, including nephritis and renal failure. Majority of studies done in Kenya have focused on pregnant women with no documented information available on the sexually active non pregnant women population despite a heightened risk of bacteriuria in this age group. The objectives of this study were to determine the occurrence of bacteriuria, to isolate bacterial pathogens associated with the bacteriuria, and to determine antibiotic susceptibility profile on the isolated bacterial pathogens in sexually active non pregnant women attending Thika Level 5 Hospital, Kiambu County, Kenya. Materials and Methods: The study employed a cross sectional design and random sampling technique. Three hundred and eighty four (384) mid-stream urine samples were collected from reproductive aged non pregnant women of attending Thika Level 5 Hospital between January 2018 to December 2018. The positive samples were cultured on Cystine Lactose Electrolyte Deficient (CLED) media and Antibiotic susceptibility test was done using muller hinton agar. Antibiotic susceptibility testing was done on the antibiotics recommended for bacteriuria by the Ministry of Health. Antibiotic susceptibility tests were analyzed using Pearson’s Chisquare test at a confidence interval of 95%. Results: Out of 384 urine samples tested, 311 (81%) samples had significant growth (P = 0.001) and 73 (19%) had no growth (P = 0.056). Bacterial pathogens isolated were Escherichia coli (41.48%), Staphylococcus saprophyticus (30.55%), Proteus mirabilis (13.85%), Pseudomonas aeruginosa (8.68%) and Klebsiella aerogenes (5.47%). Among the ten antibiotics tested, bacterial isolates were sensitive to Cefuroxime at 93.2% (χ.....enArticle