Gitahi, Stephen MainaMuthee, Eunice WothayaNgugi, Mathew PieroMachocho, Alex Kingori2025-10-012025-10-012025-08Gitahi, S. M., Muthee, E. W., Ngugi, M. P., & Machocho, A. K. (2025). Phytochemical Profile and Assessment of In Vivo Anti‐Inflammatory Efficacy of Ficus sycomorus L.(Moraceae) Extracts in Mice. Scientifica, 2025(1), 8849948.https://doi.org/10.1155/sci5/8849948https://ir-library.ku.ac.ke/handle/123456789/31488ArticleInfammation helps the immune system identify and eliminate disease-causing and foreign stimuli and initiate the healingprocess. Nonsteroidal anti-infammatory drugs have been often used in treating infammation. Synthetic drugs have been as-sociated with severe efects, necessitating the need for alternative medicinal agents. Herbal remedies have comparatively fewer sideefects, are widely available, and are arguably afordable, which makes them more attractive therapeutic agents. Ficus sycomorus isutilized by Kenya’s Mbeere community to treat infammation. However, the science-based data to support their claim werelacking. Te purpose of this study was to ascertain whether methanol (MeOH) and dichloromethane (DCM) leaf and stem barkextracts of F. sycomorus have anti-infammatory qualities in mice. Gas chromatography–mass spectrometry (GC–MS) andliquidchromatography–mass spectrometry (LC–MS) were used in phytochemical analysis of the extracts. In anti-infammatory assays,6–7-week-old mice were randomly grouped into six clusters with fve mice each. Group one mice were administered normalsaline. Groups of two to four mice were injected with carrageenan to induce infammation and then received various treatments.Group two mice received the vehicle (normal saline), while group three mice received diclofenac (15 mg/kg body weight [bw]). Teextracts were administered to the remaining groups at 50, 100, and 200 mg/kg bw. One-way analysis of variance was used to assessfor statistically signifcant diferences, followed by Tukey’s post hoc tests in case of statistical signifcance. Te signifcancethreshold was inferred at p < 0.05. Tis study revealed signifcant anti-edema efects of the extracts on carrageenan-induced pawinfammation in mice. Te GC–MS analysis identifed fatty acids, terpenoids, and terpenes, which have been associated with anti-edema efects. In conclusion, the fndings showed that the extracts have anti-edema efects and phytocompounds associated withthe efects. F. sycomorus extract is hence a novel candidate for developing efcacious anti-infammatory agentsenArticle