Mugwe, J. N.Danga, B. O.Verde, B.S.2014-07-072014-07-072014Scholarly Journal of Agricultural Science Vol. 4 ( 1 ), pp. 27 - 31 January , 20142276 - 7118http://www.scholarly-journals.com/sjas/archive/2014/January/pdf/Benvido%20et%20al.pdfhttp://ir-library.ku.ac.ke/handle/123456789/10304Declining in soil fertility continues to affect food production by affecting soil and crop productivity in the Central Highlands of Kenyan (CHK). One of the major components and drivers of soil fertility is the soil microbial biomass which contributes nutrients released through organic matter decomposition. Soil acidity contributes for reduced microbial biomass, fertility and crop yields. To evaluate the influence of manure, lime, P fertilizer and their combination on the soil microbial biomass an experiment was conducted in Embu-ATC comprising 8treatments. The study included manure (0, 5 and 10 t ha-1), Lime (0 and 2 t ha-1) and P fertilizer (0, 30 and 60 kg P ha-1). The experiment was laid out as RCBD with 4 replicates in plots of 4x4.5m. Soil samples were collected at harvest and subjected to microbial biomass analysis. The microbial biomass was analyzed by the chloroform fumigation and extraction method. Data generated was analyzed by use of SAS and LSD was used to separate means at 95% of confidence level. The results showed that application of manure, lime or their combination plus P fertilizer contributed to increased soil conditions and supplied nutrients for microorganism’s survival enhancing therefore soil microbial biomass N. Subsequently it was concluded that application of manure and lime are required in the CHK to promote microorganism’s development.enManureLimeP fertilizersoil microbial biomassArticle