Prevalence and Antimicrobial Resistance Profles of Salmonella Species and Escherichia Coli Isolates from Poultry Feeds in Ruiru Sub-County, Kenya
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Date
2021-02-02
Authors
Ngai, Dorica Gakii
Nyamache, Anthony Kebira
Ombori, Omwoyo
Journal Title
Journal ISSN
Volume Title
Publisher
BMC
Abstract
Objectives: Contaminated poultry feeds can be a major source of E. coli and Salmonella infections in poultry. This
study aimed at determining microbial load, prevalence and antimicrobial resistance profles of Salmonella sp. and E.
coli and associated resistance genes among isolates from poultry feeds.
Results: A total of 150 samples of diferent poultry feed types were randomly collected from selected sites within
Ruiru Sub-County. The microbial load was determined, Salmonella sp. and Escherichia coli were isolated and antimicrobial susceptibility test carried out. Antimicrobial resistance genes were also screened among the resistant isolates. Out
of analyzed samples, 58% and 28% contained Escherichia coli and Salmonella sp. respectively. Bacterial load ranged
between 3.1×105
and 3.0×106 cfu/g. Highest resistance was against ampicillin (41%) for Salmonella sp. and (62%)
for E. coli isolates. Ampicillin resistant isolates carried TEM and SHV genes. In addition, strB and Dfr resistance genes
associated with streptomycin and cotri-moxazole were detected. All the isolates were susceptible to chloramphenicol
and ciprofoxacin. The study reveals high bacterial contamination, presence of beta-lactamase, aminoglycoside and
sulphonamide resistance genes across isolates from poultry feeds. Therefore, contaminated poultry feeds with bacteria are likely to lead to increase in antimicrobial resistant strains across the community.
Description
Article
Keywords
Poultry feed contamination, Salmonella, E. coli, Antimicrobial resistance, Resistance genes
Citation
Ngai, D. G., Nyamache, A. K., & Ombori, O. (2021). Prevalence and antimicrobial resistance profiles of Salmonella species and Escherichia coli isolates from poultry feeds in Ruiru Sub-County, Kenya. BMC research notes, 14(1), 1-6.