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dc.contributor.authorMakang' a, Omache Benard
dc.date.accessioned2013-03-20T12:27:27Z
dc.date.available2013-03-20T12:27:27Z
dc.date.issued2013-03-20
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/6526
dc.description.abstractTicks are haematophagous ectoparasites capable of transmitting diseases to vertebrates and, therefore, constitute a threat to human, livestock and wildlife health. Though synthetic chemical aearicides have made a tremendous impact over the years in the control and management of the vector on livestock, ticks have developed resistance to most of them. In addition, the chemicals are toxic to non-target organisms. In some parts of Kenya, powdered parts of some plants, including those of Tagetes minuta L., are used to control ticks from the animals. The study aimed at characterizing and evaluating the repellency of the essential oils of T. minuta obtained from three agro-ecological zones (Nairobi-Kasarani, Westem-Bungoma and Nyanza-Bondo) against R. appendiculatus Neumann 1901. The essential oils were isolated by hydrodistillation and analyzed by gas chromatography-linked mass spectrometry (GC-MS). The constituents were identified by comparing their mass spectra with those in the National Institute of Standards and Technology (NIST) libraries cinfirmed by co-injections wth standards. The repellency of the essential oils, selected constituents and blends was evaluated using tick climbing assay. The yields of the essential oils varied in the three agro-ecological zones: Nairobi-Kasarani•0.04S±0.00S % w/w, Western-Bungoma, 0.039±O.OOS % w/w and Nyanza- Bondo, 0.03S±0.00S % w/w. That from Nairobi-Kasarani showed the highest repellency (70.06±2.76), followed by that from westem-Bungoma (60.S7±2.74) and Nyanza-Bondo (53.26±3.81). The compounds characterized from the oils were mainly monoterpenoids with some sesquiterpenoids. The major constituents were: β-ocimene (13.17 %), guaiacol (13.13 %), allo-ocimene (10.lS %), trans-tagetone (9.39 %), dihydrotagetone (8.20 %) and limonene (6.2S %) in Nairobi-Kasarani oil;, 2-pentanone (2S.28 %), allo-ocimene (13.89 %), dihydrotagetone (8.73 %), β-ocimenone (S.24 %) and trans-tagetone (4.S9 %) in Westem-Bungoma oil; and, dihydrotagetone (9.46 %), 8,9-dehydrocycloisolongifolene (6.31 %), limonene (4.77 %), caryophyllene oxide (4.21 %) andtrans-3,S-dimethyl-1,6- octadiene (2.66 %) in Nyanza-Bondo oil. Of the individual constituents and blends assayed, guaiacol and a blend of guaiacol with alloocimine were most repellent (p ≤0.05, SNK). These results confirm the scientific basis of the traditional use of T. minuta to control the ticks and lay down the groundwork for more comphensive study of local plants for the development of eco-friendly and affordable tools for managing these ectoparasites.en_US
dc.description.sponsorshipKenyatta Universityen_US
dc.language.isoenen_US
dc.titleComposition and repellency of essential oils of tagetes minuta from different zones in Kenya against brown ear tick (rhipicephalus appendlculatusien_US
dc.typeThesisen_US


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