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dc.contributor.advisorEliud NM Njagien_US
dc.contributor.advisorJoseph JN Ngeranwaen_US
dc.contributor.authorMuresia, Wafula Benedict
dc.date.accessioned2024-02-01T12:57:51Z
dc.date.available2024-02-01T12:57:51Z
dc.date.issued2023
dc.identifier.urihttps://ir-library.ku.ac.ke/handle/123456789/27407
dc.descriptionA Thesis Submitted in Partial Fulfilment of the Requirements for the Award of the Degree of Master of Science in Biotechnology in the School of Pure and Applied Sciences of Kenyatta University, July 2023.en_US
dc.description.abstractWounds are injuries which arise when the structural continuity of a tissue is physically disrupted. It has been established scientifically that plants produce metabolites which potentially accelerate wound healing. Approximately 80% of human population globally, according to WHO, use phytoextracts that have not been scientifically accredited as medicines. This therefore implies that little if nothing is known about their potential lethality. The use of such remedies has either led to fatalities or left many people hospitalized. This research therefore focused on establishing in vivo the efficacy and toxicity of lyophilized leaf aqua-extracts of M. pyrifolia, F. africana, A. conyzoides, L. ukambensis and A. oleracea in enhancing cutaneous wound healing in Mus musculus, male albino mice. Leaves of the above plants identified and confirmed by taxonomists were collected from their undisturbed natural ecosystem, dried under the shade and ground to powder using a mill. Extraction of phytoextracts was done by infusion which involved the addition of 500g of the powder to 4 liters of distilled water and maintaining the mixture at 600C for 6 hours. The mixture was filtered, concentrated, lyophilized and stored in lightproof plastic bags at -100C for bioassays. The phytoextracts were assayed for their activity in the excision wound repair paradigm. Mineral ion constitution of the extracts was analyzed using TXFR technique. The presence of phytochemical composition was established using standard procedures. For each specimen plant, the animals were put into five groups, each consisting of five mice. Group A, a negative control, was given physiological saline. Group B, a positive control, was treated with a standard drug Flucloxacillin at a therapeutic dose of 40 mg/kg and groups C, D and E with test phytoextracts at therapeutic doses 50, 95 and 300mg/kg body weight respectively. This was done systemically via oral routes for 21 days. Acute toxicity assessment was based on Organization for Economic Co-operation and Development Guideline 423 (OECD, 1996). Sub-acute toxicity evaluation focused on relative organ weight, hematological and biochemical parameters. Statistical analyses were done by ANOVA followed by Tukey HSD post hoc test. P≤0.05 was considered statistically significant. From the results, lyophilized extracts significantly accelerated wound contraction in the first five days post wounding in respect of the physiological saline control doses. Also, there was neither death nor moribund state of mice during the study period. Generally, the hematological and biochemical parameters were not significantly affected. Results from TXFR revealed the presence of mineral elements like magnesium that enhance wound healing. Phytochemical analysis results revealed the presence of the metabolites flavonoids, tannins, phenols, alkaloids and saponins in varying concentrations. In conclusion, the extracts are non-toxic and demonstrated wound healing potential which would be attributed to the presence phytochemicals and mineral ions in the phytoextracts. This research therefore recommends the sustained use of these plant extracts for wound healing purposes.en_US
dc.description.sponsorshipKenyatta University and Kenya National Research Fund.en_US
dc.language.isoenen_US
dc.publisherKenyatta Universityen_US
dc.subjectTherapeuticen_US
dc.subjectToxicological Effectsen_US
dc.subjectPhytoextractsen_US
dc.subjectCutaneous Wounden_US
dc.subjectHealing Potentialen_US
dc.subjectBungomaen_US
dc.subjectKakamegaen_US
dc.subjectKenyaen_US
dc.titleTherapeutic and Toxicological Effects of Phytoextracts with Cutaneous Wound Healing Potential in Bungoma and Kakamega Counties of Kenyaen_US
dc.typeThesisen_US


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