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dc.contributor.authorMakilla, Davies Mukwabi
dc.date.accessioned2021-02-16T11:53:19Z
dc.date.available2021-02-16T11:53:19Z
dc.date.issued2020
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/21475
dc.descriptionA Thesis Submitted In Fulfillment of the Requirement For The Award of the Degree of Doctor of Philosophy (Fisheries) In The School of Pure and Applied Sciences, Kenyatta Universityen_US
dc.description.abstractIn Kenya, fish farming has been expanding in recent years, with Government support through financial and input subsidies to small scale farmers. The sub sector generates a variety of benefits such as food and nutrition and develops trade. However, the sub sector faces the challenges of fish diseases, given that the fish production facilities support high-density living conditions that are favorable for spread of parasitic diseases. Besides, when fish that is infected is consumed by humans, the humans may be infected, especially when eaten fresh. Infected fish lowers their market value and sunctions may also be imposed on fish exports. Knowledge of fish parasites in aquaculture systems as well as treatment options available would enlighten fish farmers on the specific actions to take in order to harvest quality fish. The study was cross sectional carried out in Bungoma County from August 2015 to Decemebr 2017. The main objective of the study was to characterize and evaluate control options of pathogenic parasites present in fish farming systems in Bungoma County. Bungoma County was chosen for this study because it is one of the Counties that has high potential for fish farming. The County hosts one out of the public fish farms and hatcheries at Chwele besides authenticated private fish hatcheries. He hatcheries are sources of fish fingerlings as well as training and demonstration centres for fish farmers. At every fish pond, six water quality parameters were assessed using a portable auto sampler. Farmed Nile tilapia was randomly sampled to determine the pathogenic parasites and bacteria present in various organs. Commercial fish feeds were also purchased from two fish feed millers in Bungoma County and assessed for parasite presence. Polymerase Chain Reaction (PCR) and genetic sequencing analysis were conducted in order to assess the genes responsible for antimicrobial resistance. The data was entered into Micro Soft Excel (Windows 10) spreadsheet and analysed by SPSS Software. It was found that there was significant difference only in temperature of the pond water sampled across the sub counties (F=15.5; df=5; p<0.001). Helminthes recovered were Pallisentis tetraodontis, Pallisentis spp., Acanthocephalus spp., Procamallanus spp. and Philometroides spp. from Nile tilapia and Cleidodiscus spp. from pond water. Bacteria that were isolated from Nile tilapia were Vibrio vulnificus, Vibrio parahaemolyticus, Aeromonas hydrophila, and Pseudomonas aeruginosa. In fish pond water, Aeromonas hydrophila was isolated while Streptococcus iniae was isolated from fish feeds. Besides, there was a significant difference in distribution of total viable aerobic colony counts in fish ponds water across the different sub counties (H=6;df 5; P< 0.041). Furthermore, it was found that there was significant difference in the distribution of Aeromonas hydrophila in fish ponds across the different sub counties (H=3;df;P< 0.016). The external parasites were eliminated by formalin (36% formaldehyde) at concentration of 25ppm for 1h and Hydrogen peroxide at 75ppm for 30 min. The bacterial isolates were found to be significantly different in responses to antimicrobial agents (OLR;df= 16; P<0.00). When the bacterial isolates were subjected to PCR, all five bacterial pathogens isolated from fish, pond water and fish feeds were found to contain blaTEM gene amplified at 424bp. The sequences blaTEM gene returned 100% identity with GenBank Accession number; BankIt2236899 Seq1a MN114035 to Seq 9b MN114052). This study found that the blaTEM gene was present in recovered Vibrio parahaemolyticus, Aeromonas hydrophila, Pseudomonas aeruginosa and Streptococcus iniae. It is recommended that Formalin and hydrogen peroxide as well as potassium permanganate should used to manage fish helminthes in the pondsen_US
dc.language.isoenen_US
dc.publisherKenyatta Universityen_US
dc.subjectPathogenic Parasitesen_US
dc.subjectOreochromis Niloticus Linnaeus 1758en_US
dc.subjectFish-farmingen_US
dc.subjectBungoma Countyen_US
dc.subjectKenyaen_US
dc.titleCharacterization and Control of Pathogenic Parasites in Nile Tilapia (Oreochromis Niloticus Linnaeus 1758) in Fish-farming Systems in Bungoma County, Kenyaen_US
dc.typeThesisen_US


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