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dc.contributor.authorKinuthia, G.K.
dc.contributor.authorKabiru, Ephantus W.
dc.contributor.authorGikonyo, N.K.
dc.contributor.authorIngonga, J.M.
dc.contributor.authorKigondu, E.M.
dc.contributor.authorAnjili, C.O.
dc.date.accessioned2014-07-14T08:29:16Z
dc.date.available2014-07-14T08:29:16Z
dc.date.issued2014-04-29
dc.identifier.citationAfrican Journal of Health Sciences. 2014; 27(2):118-133]en_US
dc.identifier.issn1022-9272
dc.identifier.urihttp://www.ajhsjournal.or.ke/?p=2350#more-2350
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/10380
dc.description.abstractLeishmania major is a protozoan parasite that causes cutaneous leishmaniasis and the standard drugs are expensive and toxic. Cheaper and safer natural drugs are therefore needed. In this study, the in vitro efficacy of crude extracts of callistemon citrinus were tested against l. Major. Controls were anti leishmanial drugs pentostam and liposomal amphotericin b. The minimum inhibitory concentrations of c. Citrinus crude aqueous and methanolic extracts were 5mg/ml and 1mg/ml respectively compared to 12.5µg/ml and 6.25µg/ml for pentostam and liposomal amphotericin b respectively. The ic50 for c. Citrinus extracts against promastigotes ranged from 297.75 to 572.69µg/ml compared to 0.26 and 0.82µg/ml for pentostam and liposomal amphotericin b. The ic50 for c. Citrinus extracts against vero cells ranged from 467µg/ml to 1314.65µg/ml. The promastigotes’ viability after treatment with aqueous and methanolic extracts was 69.58% and 75.74% respectively. At 125µg/ml, the aqueous and methanolic c. Citrinus extracts had in vitro amastigotes’ infection rates (irs) of 77.0±2.50 % and 77.5±3.50% respectively. The multiplication indices (mis) and irs of amastigotes treated with c. Citrinus crude aqueous extracts and those treated with crude methanolic extracts differed insignificantly (p > 0.05). C. Citrinus methanolic extracts stimulated production of about 20µm nitric oxide in balb/c mice peritoneal macrophages suggesting immuno-modulatory role of the extracts. The crude aqueous and methanolic extracts of c. Citrinus were therefore concluded to be relatively less toxic and possessed in vitro anti-leishmanial activity against l. Major promastigotes and amastigotes.en_US
dc.language.isoenen_US
dc.publisherAfrican Forum for Health Sciencesen_US
dc.subjectCallistemon citrinusen_US
dc.subjectcrude extractsen_US
dc.subjectLeishmania majoren_US
dc.subjectcutaneous leishmaniasisen_US
dc.subjectantileishmanial activityen_US
dc.subjectvero cellsen_US
dc.titleIn vitro activity of aqueous and methanol extracts of Callistemon citrinus (Family Myrtaceae) against Leishmania major.en_US
dc.typeArticleen_US


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