Screening of media for mass micropropagation of hops (Humulus Lupulus L.) using tissue culture techniques
Ndeda, Sylvester Opil
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Hops (Humulus lupulus L.) is mainly propagated through cuttings or layering. However, the use of cuttings or layering requires a lot of time and space. Because of this, the application of tissue culture techniques to hops propagation was investigated. This investigation involved culturing explants from different hops varieties on modified media. Murashige and Skoog's medium, Probasco and Winslow's medium and Whites medium were employed. Internodes, lamina, nodes, patioles and shoot tips were used as sources of explants. Effects of Kinetin and Benzylaminopurine on shoot regeneration were also investigated. Nodes were found to be the best sources of explants for production of multiple shoots. Upto six shoots were obtained from them when Murashige and Skoog's medium was supplemented with benzylaminopurine. Shoot tip explants developed a maximum of three shoots. However, these shoots did not growth beyond 0.5cm. No shoots were obtained from leaf explants. Callus developed from the bases of most explants but further development into plantlets was not observed. Murashige and Skoog's medium gave more shoots than other media tested. Between four and six shoots were obtained with Murashige and Skoog's medium while only one or two shoots were obtained with Probasco and Winslow's medium. No plantlets grew from explants inoculated on White's medium. Auxillary bud growth started on the second week and at the end of the fourth week shoots were ready for rooting. Rooting of shoots was achieved on half-strength Murashige and Skoog's medium with either 0.2mg/1 naphthalene acetic acid or without it. However, more vigorous root growth was achieved on half-strength Murashige and Skoog's medium supplemented with 0.2mg/1 naphthalene acetic acid. Rooting started on the second week after sub-culturing and at the end of the fourth week, the plantlets were ready for transplanting.
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