Reference values for some routinely analysed biochemical parameters for adult population in North- Rift Valley, Kenya
Juma, Atieno Alice
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Clinical chemistry reference ranges vary due to factors such as age, sex, race, diet, climate, altitude and genetics. Because of this, International Federation of Clinical Chemists recommends that each laboratory establishes its own references ranges for the biochemical parameters and not rely on the Manufacturer's reference range values. There is little information in the literature on biochemical reference values for adult in Kenya and no reports on the North Rift Valley region residents. The aim of this study was to determine age and sex based reference ranges for 25 biochemical parameters for the adult population of North Rift Valley region. This was a population based cross-sectional study carried out at Moll Teaching and Referral Hospital in collaboration with the Regional Blood Transfusion Center, North Rift. A total of 400 volunteer blood donors were screened and 367 (211 males and 156 females) who were free from human immunodeficiency virus, hepatitis B and C virus, and syphilis W ere included in the analysis. Cobas Integra© 400 plus (Roche Diagnostics, Mannheim- Germany) was used to analyze the 25 clinical chemistry parameters. Clinical and laboratory Standards Institute guidelines were followed to create study consensus intervals Reference ranges were constructed using non-parameterc methods to estimate 2.5 and 97.5 percentiles of distribution as lower and upper reference limits respectively. There were significant sex differences in the North Rift Valley Kenyan reference ranges for total protein, albumin, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, total and direct bilirubin, creatinine, potassium, sodium, chloride, uric acid, amylase, lipase, creatine kinase, gamma glutamyltransferase, triglycerides, cholesterol, and low density lipoprotein. There were also significant age differences in the reference ranges for alkaline phosphatase, alanine aminotransferase, creatinine, chloride, uric acid, glucose, calcium, gamma glutamyltransferase, lipase, cholesterol, low density lipoprotein and triglycerides. The developed North Rift Valley Kenyan clinical chemistry reference ranges differ from the American values commonly used in Kenyan Hospitals. In conclusion, the findings of this study provide adult sex and age specific reference range values to be used in North Rift Valley region. This study recommends adoption of these reference ranges in North Rift and determination of similar values for other regions in Kenya.