Spectroelectrochemical Characterization Of Anti-Schistosoma-Gold Nanoparticle Conjugate For Use In Immunoassays
MetadataShow full item record
Gold nanoparticles(AuNPs) have been used widely in biomedical diagnostic and imaging. Their high stability, ease of characterization and ability to bind with biological molecules are among the properties that promote their diverse applications. Of interest is the application as electrochemical markers especially in immunohistochemistry, biosensors and immunoassays. In order to serve that role, an antibody needs to be attached to the surface of the nanoparticle since proteins readily bind to AuNPs. However, this immobilization process might result in nanoparticle aggregation or the loss of the bioactivity of the conjugated antibodies. We hereby report the optimization of the covalent binding of anti-schistosome antibody to AuNPs via a carbodiimide cross-linker The characterization and functionality testing of the conjugation was done using UV/Vis spectrometry and electrochemically by cyclic voltammetry(CV). The conjugate was tested with varying concentrations of Soluble Egg Antigen (SEA) as the analyte. The signal response was directly proportional to the concentration of SEA. A plot of concentration of SEA against the change in current gave a limit of detection of 3.31×10-5 ng/ml based on 3 times the standard deviation of the blank. These findings demonstrated the potential for development of a point-of-care immunosensor for rapid diagnosis of Schistosomiasis.