Comparative Sensitivity of Widal Test, Stool and Blood Culture as Diagnostic Methods for Salmonella typhi Infection
Typhoid is one of the infectious diseases of human. Outbreaks of typhoid fever caused by Salmonella typhi are still a serious health problem worldwide. There are a number of test available presently, from molecular to immunological and biochemical to microbiological. This study will compare the sensitivity of Widal test, stool culture and blood culture as diagnostic methods for Salmonella typhi infections. Both the tube and slide agglutination Widal test will be used to investigate the incidence of typhoid fever in two hundred and fifty typhoid fever patients attending Chuka General Hospital. The Widal tube agglutination test will be done first on each blood sample so as to determine the Widal titre level before performing the Widal slide agglutination test. This will help in proper interpretation of the result of the slide agglutination test which is most used in health care laboratories in Kenya. All samples will be analyzed for typhoid fever infection using both stool and blood cultures from the same individuals. Sensitivity (true-positive rate) will be defined as the probability that the Widal test result will be positive when blood culture confirms that typhoid fever is present. To compare the sensitivities of the three diagnostic test methods, data will be transferred onto SSPS spreadsheets for processing. Data will be entered and filtered from the spreadsheet, analyzed and presented in form of tables, graphs and pie charts. The findings from this study will provide valuable information to policy makers and health personnel on the most sensitive and reliable method for routine diagnosis of typhoid fever health care centers.