In vitro effects of five plant herbs used to treat retained Placenta in Vihiga County on rabbit's uterine stripe
Ihiro, Batroba Vulogosi
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The practice of obstetrics is an important aspect of the traditional health care delivery system of most cultures in Africa. In spite of the influence of western culture, this practice still thrives especially among rural population mainly due to the high cost of modem medicine, level of awareness and availability of the service. Women whose delivery is assisted by a trained health care provider are less likely to suffer as a result of birth complications. One of the causes of postpartum death is retained placenta. A retained placenta is where it is not expelled within 30 minutes after birth and it prevents the contractions of the uterus which results into excessive bleeding. In Vihiga County some women deliver at home assisted by relatives or traditional birth attendants who often use herbal remedies in the process which include Cucurbita pepo, Ricinus communis, Trimfetta rhomboidea, Cleome gynandra and Sida tenuicarpa. However scientific studies have not been done to establish the effectiveness of these plant extracts. The aim of this study is to determine the effectiveness and dose of the five herbs used in treatment of retained placenta in vitro using a rabbit's uterus. The roots of the five plants will be collected, botanically identified, dried, crushed into powder and soaked in dichloromethane methanol mixture at the ratio 1:1 for 72 hours. The extract will be dissolved in 5% dimethyl sulphoxide in normal saline and three doses of each will be used in the experiment. The effectiveness of the plant extracts will be determined by measuring the contractions initiated on an isolated rabbit's uterine stripe using an organ bath connected to a kymograph with a rotating drum. The intensity and frequency of the contractions will be extrapolated as the effects produced by the plant extracts on a human uterus. Oxytocin and ergometrine will serve as positive controls while distilled water will serve as a negative control. Data obtained will be tabulated and analyzed with analysis of variance and Dunnets test as a post hoc test. A value of p <0.05 will be considered significant.